Figure 2: Sensitive and specific prion seeding activity from organotypic slice cultures on RT QuIC assay.(A) Slice cultures from WT mice (C57BL/6 background) efficiently seed the RT QuIC assay. Representative RT QuIC responses shown for the reactions seeded with 5ng of WT homogenates from either NBH or RML scrapie infected slice cultures (21 dpi) or brain homogenates from mock or terminally infected scrapie mice. Uninfected samples did not show seeding activity and stayed at the baseline throughout the reaction.
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